How to Do a Faecal Worm Egg Count
Faecal worm egg count (FWEC) is a crucial diagnostic tool used to detect and monitor the presence of parasitic worms in animals, particularly in livestock and pets. This test helps in determining the level of worm infestation and assists in making informed decisions regarding deworming treatments. If you are a veterinarian, a farm manager, or simply a pet owner looking to understand how to perform a faecal worm egg count, this article will guide you through the process step by step.
Materials Needed
Before you begin, gather the following materials:
1. Faecal sample collection kit: This kit usually includes a clean, sterile container, gloves, and a label for the sample.
2. Microscope: A compound microscope with a 10x objective lens is typically sufficient for conducting the FWEC.
3. Microscope slides and cover slips: These are used to prepare the faecal samples for examination.
4. Haematoxylin and eosin (H&E) stain: This stain is used to enhance the visibility of worm eggs under the microscope.
5. Scissors and a ruler: These are necessary for measuring the faecal sample.
Collecting the Faecal Sample
To collect the faecal sample, follow these steps:
1. Wear gloves to prevent contamination.
2. Use the sterile container provided in the kit to collect the faecal sample.
3. Collect a fresh sample directly from the animal, ensuring that it is not mixed with urine or bedding material.
4. Label the container with the animal’s name, date, and any other relevant information.
5. Place the container in a cool, dry place until it is ready for analysis.
Preparation of the Sample
Once you have collected the faecal sample, prepare it for examination:
1. Place a microscope slide on a flat surface.
2. Using a clean, disposable scalpel, cut a small piece of the faecal sample.
3. Place the faecal sample on the slide and cover it with a cover slip.
4. Gently press the cover slip to spread the sample evenly across the slide.
5. Use the ruler to measure the faecal sample and ensure it is within the recommended range (usually 0.1 to 0.5 grams).
Staining the Sample
To enhance the visibility of worm eggs, stain the faecal sample:
1. Place the stained slide under the microscope.
2. Use the 10x objective lens to examine the sample.
3. Look for worm eggs, which may appear as small, round or oval-shaped structures with a clear nucleus.
Counting the Worm Eggs
To count the worm eggs, follow these steps:
1. Identify the worm eggs in the sample.
2. Count the number of eggs within the measured area of the slide.
3. Record the count and calculate the worm egg count per gram (EPG) by multiplying the number of eggs by the conversion factor (usually 1000).
Interpreting the Results
The FWEC results can help determine the level of worm infestation and guide deworming treatments. Here are some general guidelines for interpreting the results:
– EPG < 200: No treatment is necessary. - EPG 200-500: Consider deworming treatment. - EPG > 500: Deworm the animal immediately.
Remember that these guidelines may vary depending on the species and the specific worms involved. Consult with a veterinarian or a farm advisor for more personalized advice.
In conclusion, performing a faecal worm egg count is a simple yet essential process for monitoring and managing worm infestations in animals. By following these steps, you can ensure accurate results and make informed decisions regarding deworming treatments.
